NMR reveals linkage-specific conformational ensembles of non-canonical polyubiquitin chains
نویسندگان
چکیده
1 Dept. of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA; 2 Depts. of Biology and Chemistry, Syracuse University, Syracuse, NY 13244, USA; 3 Institut des Sciences Analytiques, UMR5280-Université de Lyon, 69100 Villeurbanne, France; 4 Dept. of Chemistry, Virginia Commonwealth University, Richmond, VA 23284, USA; 5 NIST Center for Neutron Research, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
منابع مشابه
Linkage-specific conformational ensembles of non-canonical polyubiquitin chains.
Polyubiquitination is a critical protein post-translational modification involved in a variety of processes in eukaryotic cells. The molecular basis for selective recognition of the polyubiquitin signals by cellular receptors is determined by the conformations polyubiquitin chains adopt; this has been demonstrated for K48- and K63-linked chains. Recent studies of the so-called non-canonical cha...
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The conformational flexibility of linkage-specific polyubiquitin chains enables ubiquitylated proteins and their receptors to be involved in a variety of cellular processes. Linear or Met1-linked polyubiquitin chains, associated with nondegradational cellular signalling pathways, have been known to adopt multiple conformations from compact to extended conformations. However, the extent of such ...
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All seven lysine residues in ubiquitin contribute to the synthesis of polyubiquitin chains on protein substrates. Whereas K48-linked chains are well established as mediators of proteasomal degradation, and K63-linked chains act in nonproteolytic events, the roles of unconventional polyubiquitin chains linked through K6, K11, K27, K29, or K33 are not well understood. Here, we report that the unc...
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Polyubiquitin chains regulate diverse cellular processes through the ability of ubiquitin to form chains of eight different linkage types. Although detected in yeast and mammals, little is known about K29-linked polyubiquitin. Here we report the generation of K29 chains in vitro using a ubiquitin chain-editing complex consisting of the HECT E3 ligase UBE3C and the deubiquitinase vOTU. We determ...
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Ubiquitylation regulates a multitude of biological processes and this versatility stems from the ability of ubiquitin (Ub) to form topologically different polymers of eight different linkage types. Whereas some linkages have been studied in detail, other linkage types including Lys33-linked polyUb are poorly understood. In the present study, we identify an enzymatic system for the large-scale a...
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تاریخ انتشار 2016